The alzet osmotic pump model 2ml4 delivers solutions continuously for 28 days. Mice were housed under institutional animal care and use committeeapproved germfree conditions. Alzet pump model 1004 2001 2004 2006 duration days 28 7 28 42 pump rate. The pumping rate of the model 2ml4 alzet osmotic pump over time in vitro and in vivo in sprague dawley rats after subcutaneous implantation n105. Osmotic drug delivery system as a part of modified release. Development of pushpull osmotic pump tablets for a slightly water soluble drug piyush patel, shahrzad missaghi, sandip b. Isoproterenol concentration varies depending on the body weight of the animal due to constant flow rate. Rose and nelson developed an implantable pump in 1955. Via a catheter, alzet pumps can be used to deliver substances into the venous or arterial circulation, into the brain, or into any organ, lumen, or solid tissue. The graph on the left shows the representative pumping rates for two pump models from 4 degrees to over 40 degrees in 0. Jul 17, 2012 pushpull osmotic pump is a modification of eop figure 8. Introduce the pump into the back of the animal leaving the catheter and cannula outside figure 2b.
Eppendorf tubes to prepare solution for individual mice 0. Alzet osmotic pumps for laboratory animal dosing overview. It was introduced to the market in the early 1980s in osmosin indomethacin and acutrim phenylpropanolamine, but unexpectedly severe issues with gi irritation and cases of gi perforation led to the withdrawal of osmosin. Osmotic pumps release drug at a rate that is independent of the ph and hydrodynamics of the dissolution medium. Pirfenidone inhibits fibrocyte accumulation in the lungs. Isoproterenolinduced heart failure mouse model using. The therapeutic use of osmotic minipumps in the severe. Osmotic pump delivery system authorstream presentation.
As the core absorbs water, it expands in volume, which pushes the drug solution out. It is not to be placed into animals used for food or food products, or in humans. Continuous ophthalmic treatment using an osmotic pump in a. Products fluid handling osmotic pumps alzet osmotic pumps. Durect manufactures and sells alzet osmotic pumps and accessories on a worldwide basis. The alzet mini osmotic pump model 2001 delivers solutions continuously for 7 days without the need for external connections or frequent handling of animals.
Cd or captisol, which acted as both a solubilizer and as an osmotic agent. Convenient, reliable, and costeffective alternative to chronic injections continuous delivery ensures constant compound levels in plasma or tissues for maximised therapeutic efficacy and reduced adverse effects. This video article jove describes a method for targeted administration of drugs to the central nervous system by either continuous delivery using alzet osmotic pumps or performing bolus injections into the right lateral ventricle of mice. The core of pushpull osmotic pump tablets is composed of a drug layer containing an active drug and a push layer having expanding agents 17. This video shows the parts of the pump and describes how the pump operates to enable continuous delivery of experimental agents.
Based on data from many laboratories, there have been consistent findings that this is a reliable and reproducible method to study both aaas 3,4 and taas 2226 in mice. Implantation of miniosmotic pumps and delivery of tract. Alzet pumps ensure that test compounds are present in plasma and tissues for a sufficient duration to allow their biological effects to develop fully and reproducibly. Osmotic pumps can be used for systemic administration. The alzet osmotic pump model 2ml2 delivers solutions continuously for 14 days. Constant zeroorder release is maintained as long as the drug solution is saturated. Alzet surgical implantation procedures video on vimeo.
This video provides an overview of the alzet technology, benefits, and applications. In this research, rats were implanted with 7day minipumps model 2001 containing 24 mgkgday pyridostigmine, and blood samples were taken at intervals from 5 to 60 min after implantation. The measuring cylinder containing a known mass of granules blend is tapped. Demonstrated procedures include subcutaneous and interperitoneal implantation, and the use of a catheter for intravenous jugular, intrathecal, intragastric, and cranialcerebral infusion. Implantation of the alzet miniosmotic pump in vivo. It is a selfcontained intelligent device consisting of a microcontroller with associated memory, embedded software, inputsoutputs, a micro pulse stepping motor, crystal oscillators.
Osmotic drug delivery uses the osmotic pressure for controlled delivery of drugs by using osmogens for upto 10 16 hrs. Osmotic pumps are miniature infusion pumps for the continuous dosing of laboratory animals. Following the surgical procedure of pump implantation, a temporary delay in body weight gain was observed. Alzet osmotic pumps are miniature, implantable pumps used for research in mice, rats, and other laboratory. Osmotic pumps such as those supplied by alzet can be connected to a catheter. Rajabisiahboomi1over the last decade, there has been increasing interest in the development of oral osmotic devices in which the. Durect corporation alzet osmotic no 1004 pump alzet. Alzet pumps can also be used for targeted delivery, where the effects of a drug or test agent are localized in a particular tissue or organ, by means of a catheter. Subcutaneous angiotensin ii infusion using osmotic pumps induces aortic aneurysms in mice article pdf available in journal of visualized experiments 2015103 october 2015 with 207 reads. The osmotic delivery device is based on higuchitheeuwes pump design figure 6. Nine alzet pump models, in a range of release rates and durations, are appropriate for use in mice. Pdf gradient generation by an osmotic pump and the. It became available for investigational use in the mid1970s, and researchers used it routinely in the screening of active agents, animal toxicology and pharmacology, and initial clinical testing, creating. Such osmotic pump tablets contain leachable watersoluble components in their membranes, thus delivery orifices are formed when the watersoluble components dissolve.
Infusion pumps can administer fluids in ways that would be impractically expensive or unreliable if performed manually by nursing staff. Pdf subcutaneous angiotensin ii infusion using osmotic. Maximize therapeutic efficacy and reduce adverse effects. Implantable drugdispensing osmotic pump, shaped as a small rod with titanium housing. Another kind of osmotic pump tablet suitable for the delivery of watersoluble drugs is the porosity osmotic pump tablet verma et al. The brochure design and layout needed to produce a strong first impression and present alzet as a market leader. It is generally used intravenously, although subcutaneous, arterial and epidural infusions are occasionally used. A new electroosmotic pump based on silica monoliths.
Alzet resource videos osmotic pump mechanism on vimeo. Osmotic pumps delivering angii subcutaneously is a routine approach to inducing aortic aneurysms in mice. Controlled porosity osmotic pump is an osmotic tablet in which membrane contains. Miniature infusion pumps for mice deutsche messe ag. Complete osmotic pump system with flow moderator in place. Durect a biopharmaceutical company, acquired alzet osmotic. They offer a simple, convenient and reliable alternative to repetitive videos in alzet osmotic pumps on vimeo.
Alzet pumps deliver by the process of osmotic displacement. Through osmosis, water from the body is slowly drawn through the semipermeable membrane into the pump by osmotic agent residing in the engine compartment, which expands the osmotic agent and displaces a piston to. Colligative property is the concentration of solution independent of solute property. The video also describes proper filling of the alzet pump and and surgical techniques for implantation in rats and mice.
Subcutaneous angiotensin ii infusion using osmotic pumps. A what is osmotic pressure osmotic pressure is a most important colligative property according to pharma point of view. Although shown to be thermodynamically feasible, the osmotic principle is not likely to be used commerically for these purposes in the near future. Pumps are to be implanted with the animal under general anesthesia. Durect reached out to dystrick to create a product sales brochure to help promote alzet osmotic pumps. Save time by eliminating the need for frequent animal handling. Small infusion pumps and alzet catheters for continuous dosing of unrestrained laboratory animals. To ensure uniform delivery, suspensions must remain homogeneous throughout the duration of delivery. In the present study elementary osmotic pump eop of highly water soluble drug tramadol hydrochloride trh was developed and evaluated.
Alzet pumps are available in a variety of sizes, durations and flow rates to meet a wide range of research needs. This system resembles a standard bilayer coated tablet. The nominal performance is a target for all pumps manufactured. Channel is long and straight electro double layer has a finite width cross section of the channel is constant along the flow direction the applied electric field is uniform and along the x axis of the channel the potential at the wall is constant and. A schematic diagram of sandwiched osmotic tablets can be seen in the fig.
May 26, 2016 this video showing osmotic pump insertion for drug delivery. Continuous drug infusion with an implantable osmotic pump. The pumping rate of the model 2002 alzet osmotic pump over time n20. While the pumping rate of each model is fixed at manufacture, the dose of agent delivered can be adjusted by varying the concentration. Alzet osmotic pumps implantable infusion pumps for. Osmotic pumps consist of an inner core containing drug and osmogens, coated with a semipermeable membrane. Osmotic, osmosis delivery, pump, solvent, alzet, telescopic, surfactant, polymer. If more than 10 pumps are needed, ensure that the lot numbers of the pumps are the same for one study, since pumps from different lot numbers have different mean fill volume and pump rate. It was the major the major mile stone in the field of oral osmotic drug delivery system. Implantable continuous infusion osmotic pumps have several applications in preclinical drug. Alzet osmotic pumps are cylindrically shaped see fig. The elementary osmotic pump eop was developed by alza in 1974, and was the first practical example of an osmotic pump based drug release system for oral use.
The imbibed water compresses the impermeable reservoir, which collapses and displaces the contents from the pump at a controlled, predetermined rate figure 2. Development and evaluation of elementary osmotic pump of. Osmotic pump drug delivery technology can also be used to deliver high drug. For instructions on how to prepare the pump for these applications, refer to section vll.
The scientific literature contains many examples where alzet pumps have facilitated full development of drug effects, including those of proteins, peptides, and other rapidly. Through continuous infusion, these pumps maintain a welldefined, consistent pattern of drug exposure throughout the duration of the experiment. Plastic test tubes for dissolution of the reagent or incubation of osmotic pumps 4 15 ml. The osmotic agent is placed between the core and a semipermeable membrane, which forms the outer surface of the pump. Individual lots of pumps may vary from this target within limits. Pdf osmotic micropumps for drug delivery researchgate. As the core absorbs water, it expands in volume, which pushes the drug solution out through the delivery ports. Alzet pumps are capable of delivering homogeneous viscous solutions with a viscosity of less than 100,000 centipoise.
Expert system for the development and formulation of push. Pdf osmotically controlling drug delivery system is one of the best method of drug. Alzet pumps operate because of an osmotic pressure dif ference between a. This video describes the inner parts of an alzet pump and how they operate to enable continuous delivery of experimental agents. Osmoticcontrolled release oral delivery system wikipedia. A filling needle is included in the box with osmotic pumps. By attaching the proper catheter, alzet osmotic pumps can deliver substances into the venous or arterial circulation, into the brain, or into any organ, lumen or solid tissue. Infusion pumps for continuous dosing of laboratory animals. Use the forceps to open the space under the skin in the back of the animal where the pump will be implanted. The use of alzet pumps in mice is documented since 1976 in over 3,600 published studies. We market the alzet product line directly in the u. Pushpull osmotic pump is delivered both poorly water soluble and highly water soluble drugs at a constant rate.
Recent advances include development of the controlled porosity osmotic pump, and systems based o. L reservoir to administer isoproterenol over 3 weeks. Durect corporation alzet osmotic pump model 1002 alzet. Complete micro osmotic pump system with flow moderator in place 1. A highpressure electro osmotic eo micro pump fabricated by a solgel process is shown to be potentially effective as a fluiddriving unit on chipscale analytical systems. If a pump instruction and specification sheet durect corporation manufactures a miniature implantable pump for use in laboratory animals. An infusion pump infuses fluids, medication or nutrients into a patients circulatory system. Alzet pumps are small, implantable devices for continuous dosing of unrestrained lab animals. Elementary osmotic pump eop b multiple chamber osmotic pump 1. The release of pdl from osmotic pump devices and tablets was. Blm was infused constantly via the pumps over 7 days according to the manufacturers instructions.
This video shows the parts of the pump and describes how the pump operates to enable continuous delivery. Convenient and reliable alternative to frequent injections. Alzet osmotic pumps are a simple, convenient and reliable method for continuous dosing of laboratory animals, including mice and rats. Dosage variability can be achieved by modifying agent concentration. Mice and alzet osmotic pumps female crlscidbr mice, 45 weeks of age, and alzet osmotic pumps model 2004 were purchased from charles river sulzfeld, germany. The release rate of a drug from an osmotic system is directly proportional to the osmotic pressure of the core formulation c. The in vitro pumping rate of the model 1003d alzet osmotic pump over time n20. This video describes the inner parts of an alzet pump and how they operate to enable continuous delivery of. Test agent effects develop fully and are easily reproduced, which is particularly advantageous for agents with short halflives.
This product is for use in experimental animals only. Premature excess release from the alzet osmotic pump. Alzet pumps have been exposed to a sterilizing dose of radiation from a 60co source. These minipumps provide researchers with a method for controlled and continuous agent delivery in vivo. Durect corporation alzet osmotic no 1004 pump alzet osmotic. The purpose of this study was to develop a controlledporosity osmotic pump tablet opt for poorly water soluble drugs using a sulfobutyl ether. One layer the upper layer contains drug in a formulation of polymeric, osmotic agent, and other tablet excipients. The alzet mini osmotic pump model 2002 delivers solutions continuously for at least 14 days without the need for external connections or frequent handling of animals. When the osmotic pump is exposed to an aqueous environment, the osmotic layer imbibes water through a semipermeable membrane which forms the outer surface of the pump. This video showing osmotic pump insertion for drug delivery. These infusion pumps continuously deliver drugs, hormones and other test agents at controlled rates from one day to six weeks without the need for external connections or frequent handling which. Scid mouse experiments as described in this study were. Technical description of the alzet micro osmotic pump model 1002 a. Alzet pumps can be implanted sc or ip to enable systemic.
To achieve an optimal zero order delivery profile, the orifice must be smaller. Development of pushpull osmotic pump tablets for a slightly. After filling the pump body with angii, the flow moderator is inserted to seal the pump. Alzet pump model 1004 2001 2004 2006 duration days 28 7 28 42. Alzet pumps have been used to target delivery to a wide variety of sites including the spinal cord, spleen, liver, organ or tissue transplants, and wound healing sites. Leeper pumps, the alzet and osmet systems, the elementary osmotic pump, and the pushpull system. Cortese et al,1982 1976 patent granted on the design of alzet osmotic pumps which later extensively used as an experimental research tool in laboratory animal. The core above finally coated with a layer of semi. Sep 16, 2015 alzet pumps deliver by the process of osmotic displacement. Alzet pumps can be used for systemic administration for targeted drug. Pdf osmotic drug delivery system as a part of modified. In vitroin vivo evaluation of nanosuspension release from.
Suspensions may be delivered from the pump if they do not precipitate. By combining the technique with a washout period, stem cell. Durect corporation 0009922pk micro osmotic pump model 1004. Prednisolone pdl was chosen as a model drug for this study. Push pull osmotic pump ppop c modified oral osmotic drug delivery systems oodds 1. Alzet pumps are drug discovery tools that offer researchers enhanced control over test compound levels in plasma and tissues. The pumping rate of alzet osmotic pumps varies in direct proportion to temperature. B operation principle of a single compartment pump. Durect corporation, cupertino, california, usa containing 200. Osmotic pumps of different sizes and drug duration are available for purchase from alzet.
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